ThesisOpen AccessMetadata saja
2026•Skripsi, Thesis, Disertasi, dan Tugas Akhir
ABSTRAK Hiu lontar (Rhynchobatus australiae) merupakan salah satu spesies Elasmobranchii yang berperan penting dalam ekosistem perairan pesisir dan berstatus Sangat Terancam Punah (Critically Endangered) menurut IUCN akibat tingginya tekanan penangkapan dan degradasi habitat. Penelitian ini bertujuan mengidentifikasi jenis dan karakter molekuler hiu lontar dari Perairan Tanah Kuning, Kabupaten Bulungan, Kalimantan Utara, menggunakan pendekatan morfologi dan DNA barcoding. Penelitian dilaksanakan pada Maret - Mei 2026 menggunakan tiga sampel yang diperoleh dari hasil tangkapan pukat hela. Analisis morfologi dilakukan berdasarkan karakter morfologi dan morfometrik, sedangkan analisis molekuler meliputi ekstraksi DNA, amplifikasi PCR, sekuensing, analisis BLAST, perhitungan jarak genetik, dan konstruksi pohon filogenetik. Analisis DNA barcoding menghasilkan panjang sekuens 602–714 bp dengan tingkat kemiripan 99,84–100% terhadap data referensi GenBank. Nilai jarak genetik antar sampel sebesar 0,000 menunjukkan hubungan kekerabatan yang sangat dekat. Analisis filogenetik membentuk dua clade, yaitu in-group yang terdiri atas sampel penelitian dan sekuens referensi R. australiae dari perairan lainnya, serta out-group yang terdiri atas Carcharodon carcharias. Hasil penelitian mengonfirmasi bahwa hiu lontar yang ditemukan di Perairan Tanah Kuning merupakan spesies Rhynchobatus australiae. Informasi genetik yang diperoleh diharapkan dapat mendukung upaya konservasi, pengelolaan, dan perlindungan hiu lontar secara berkelanjutan di Indonesia. Identification of the species and molecular characteristics of the spadenose shark from Tanah Kuning waters using DNA barcoding
ThesisOpen AccessMetadata saja
2025•Skripsi, Thesis, Disertasi, dan Tugas Akhir
The gecko shark is one of the fishery commodities caught by fishermen in the Tanah Kuning Waters, Bulungan Regency, North Kalimantan. The purpose of this study was to identify the species and molecular characteristics of the gecko shark using the mitochondrial COl gene-based DNA barcoding method. The study was conducted from December 2024 to March 2025. The method used was random sampling, with three gecko shark specimens taken from the Tanah Kuning waters using a trawl. Identification was carried out through two approaches: (1) morphological analysis using morphometric such as total length, head length, and fin shape; and (2) molecular analysis with stages of DNA extraction, DNA purity measurement, and mitochondrial COl gene amplification with PCR, electrophoresis, and DNA sequencing. Genetic identity validation was carried out using BLAST analysis and genetic distance calculations using the Tamura 3-Parameter model. The results of the nucleotide base sequence validation through the NCBI website showed that the sample had 100% similarity to Hemigaleus microstoma from Malaysia. Genetic and phylogenetic distance analysis showed that the sample from North Kalimantan was closely related to the samples from Bali and India. Based on the IUCN, Hemigaleus microstoma was categorized as a vulnerable species, so the results of this study confirmed the need for conservation efforts for this species in the study area.
ThesisOpen AccessMetadata saja
2025•
Hammerhead shark is a species of shark with a distinctive hammer-like head shape and it is known as a top predator in the marine ecosystem. Its existence is important in maintaining the balance of the food chain, but its population is threatened by excessive exploitation. The purpose of this study was to identify the types and molecular characteristics of hammerhead sharks (Sphyrna sp.) from the waters of Tanah Kuning, Bulungan Regency, North Kalimantan, using morphological and molecular approaches through the DNA barcoding method. The study was conducted from December 2024 to March 2025 through a purposive sampling method, by taking five hammerhead shark specimens using a trawl. Identification was carried out through two approaches: (1) morphological analysis including morphometric including total length, head length, and fin shape; and (2) molecular analysis through the stages of DNA extraction, purity measurement, and mitochondrial COI gene amplification with PCR, electrophoresis, and DNA sequencing. Genetic validation was carried out using BLAST analysis and genetic distance calculations using the Tamura 3-Parameter model. The results of morphological identification showed that all samples had the characteristics of the Sphyrna lewini species. Molecular validation through BLAST showed a similarity level of 99.54-100% with GenBank data for Sphyrna lewini, and genetic distance analysis indicated a very close relationship between individuals. This study proved that DNA barcoding was effective for identifying hammerhead shark species and had the potential to support conservation and sustainable management of shark resources in Tanah Kuning waters.
ThesisOpen AccessMetadata saja
2024•Skripsi, Thesis, Disertasi, dan Tugas Akhir
PENEGAKAN HUKUM TERHADAP PENYALAHGUNAAN DANA DESA DI DESA TANAH KUNING KECAMATAN TANJUNG PALAS TIMUR KABUPATEN BULUNGAN Abstrak Tindak Pidana Korupsi di Desa Tanah Kuning dilakukan oleh Pejabat Kepala Desa Tanah Kuning terbukti melakukan Tindak Pidana Korupsi Dana Desa sesuai dengan Rumusan Pasal 3 Jo. Pasal 18 ayat (1) RI Nomor 20 Tahun 2001 Tentang Perubahan Atas Undang-undang RI Nomor 31 Tahun 199 Tentang Pemberantasan Tindak Pidana Korupsi, dengan menggunakan Dana Desa untuk kepentingan pribadi sehingga perbuatan tersebut telah merugikan Desa/Negara. Penelitian ini dilakukan untuk membahas menganai penegakan hukum terhadap penyalahgunaan dana desa di desa tanah kuning kecamatan Tanjung palas timur kabupaten Bulungan, serta bagaimana Proses mekanisme tindakan penegakan terhadap penyalahgunaan dana desa yang di lakukan oleh kepala desa. metode dalam penelitian ini menggunakan penelitian normatif, hasil dari penelitian ini dapat disimpulkan, Bahwa Kepala Desa Tanah Kuning terbukti melakukan Tindak Pidana Korupsi Dana Desa sesuai dengan Rumusan Pasal 3 Jo. Pasal 18 ayat (1) RI Nomor 20 Tahun 2001 Tentang Perubahan Atas Undang-undang RI Nomor 31 Tahun 199 Tentang Pemberantasan Tindak Pidana Korupsi Kata Kunci : Dana Desa, Korupsi, penyalahgunan, tanah kuning
